NK VUE Information for Medical Professionals

 

 

The assay is essentially a standard ELISA. However, since the detectable level of IFNg is in the pg range, it is naturally more sensitive than most ELISAs who often detect nanogram or microgram levels of analyte, so we recommend training and practice before using the test.

 

Summary of Procedure

 

1.

Remove samples from refrigerator / Thaw samples if frozen

2.

If stored, centrifuge 1 minute at 11,500g

3.

Pipet standard curve and samples (50 μL each) into pre-coated microwell strips

4.

1st incubation: Capture step Then wash 4X

5.

Add antibody-enzyme conjugate (100 μL)

6.

2nd incubation: Binding step Then wash 4X

7.

Add TMB solution (100 μL)

8.

3rd incubation: Color development (in the dark)

9.

Add stop solution (100 μL)

10.

Read plate at 450 nm (and 620 nm) in ELISA reader

 

 

Here is a video for the full ELISA procedure, which can also be viewed in our resource section.

 

 

You can view our NK VUE ELISA product insert for a detailed explanation, which can also be found in our resources section.

If you are experiencing some problems, and the results don’t look as they should, you can view our NK VUE troubleshooting guide and Troubleshooting Check List to help you find potential causes. These documents can also be found in our resources section.

 

 

0