Custom Development Services

We offer custom development of recombinant proteins and monoclonal antibodies to meet the specific research needs of our customers.

DNA Cloning Service

One mutation is defined as any combination of mutation within a 3-base frame. Our delivery package includes vector containing target gene, complete sequencing data and construct maps. Prices vary according to the length of genes. If the target gene and destination vector isn’t provided by the customer, there will be additional charges. For specific pricing information, please contact us.

ServiceDeliverableDescriptionPriceTimeline
Subcloning (E.Coli) Target gene inserted in destination vector that the customer provides Sub cloning into one expression vector $540 2 weeks
Subcloning (Baculovirus) Target gene inserted in Baculovirus expression DNA (bacmid) Sub cloning into one expression vector $700 2-3 weeks
Additional service Mutagenesis Target gene inserted in destination vector after mutagenesis The first amino acid mutation (3 base pairs) is a fixed price in addition to the sub cloning fee. Please ask for a quote since different factors play a role in the price. $540~ 3 weeks

Notes:

  1. The above price list is only for genes shorter than 1.5Kb. For longer genes, we add an additional $115 per Kb for screening and sequencing.

  2. The target gene must be provided by customer. If not, we charge $1,300 per gene (in addition to the sub cloning fee) and project duration will take 8 weeks instead of the above mentioned 2 weeks. If a specific destination vector is desired, the customer has to provide it. If DNA Synthesis is required, the price is $0.50/1bp. May be subject to change, so please email us for a price quote.

  3. One mutation is defined as any combination of mutation within a 3-base frame.

  4. Our delivery package includes the vector containing target gene and complete sequencing data and construct maps.

 

 

Protein Expression & Purification Service

ATGen has a great expertise in developing recombinant proteins using Escherchia coli as a host. Our Protein services are charged according to the required amount of strain comparison and optimization work. The pricing is based on a setup fee for expression analysis, the number of different strains (possibly with different helper plasmid systems) and the number of different culture conditions tested. For specific pricing information, please contact our sales department.

 

E.Coli Expression Full Service

ServiceDeliverableDescriptionPriceTimeline
*Expression and Identification of target protein Report including SDS page image, expression levels according to E.coli strains, etc. *Non-reimbursable setup charge to determine solubility and level of difficulty. $100 1 week
Option 1
Purification
1L expression and 1-step purification. Purified soluble tagged protein and prices vary according to the expression patterns. $1,800~ 2-3 weeks
Option 2
Non-Purification (Denatured)
Preparation of protein with inclusion body in buffer. A denatured form of protein (without using conventional chromatography techniques) is provided. $1,200 2-3 weeks
Additional service
QC by WB (Ab provided by the customer)
Western blot assay image. WB performed at no extra charge, but the customer has to provide the antibody for the target protein. free 1 week
Additional service
Endotoxin detection
Report Whether the endotoxin level of target protein is above or below 1.0 EU per 1 microgram of protein is determined by LAL method. There is no guarantee that the level is < 1.0 EU per 1 microgram. $150 1 week

* Expression and Identification of target protein is a mandatory first step to determine the price of purification. This step is paid in advance and is not refundable.

** Prices for scale up and additional purification steps (such as removing endotoxin, increase purity, refolding, specific buffer changes, concentration changes, etc.) are to be determined after a 1L pilot expression and 1-step purification.

*** If both client and ATGen agree for ATGen to add target protein to ATGen’s product list, ATGen will offer a 30% discount.

 

Baculovirus Expression Full Service

ServiceDeliverableDescriptionPriceTimeline
Recombinant virus generation, protein expression, and purification Report *Non-reimbursable setup charge to determine feasibility. $200 4-6 weeks
Purified proteins and QC data. Transfection of insect cell with recombinant Bacmid DNA. $4,100 4-6 weeks
Generation of P1 stock (Low titer), P2 stock (High titer)
Insect cell expression, Infection of insect cells with P2 stock (5ml)
Purification (expression in 200ml media with 1-step purification)

* Recombinant virus generation, protein expression, and purification is a mandatory step to determine the price of purification. This step is paid in advance and is not refundable.

** Prices for scale up and additional purification steps (such as removing endotoxin, increase purity, refolding, specific buffer changes, concentration changes, etc.) are to be determined after a 200mL pilot expression and 1-step purification.

*** If both client and ATGen agree for ATGen to add target protein to ATGen’s product list, ATGen will offer a 30% discount.

 

 

Custom Monoclonal Antibodies

ATGen’s monoclonal antibodies are produced in hybridoma cell lines derived from the fusion of myeloma cells and antibody-producing B-lymphocytes. We guarantee two positive clones for monoclonal antibodies developed in mouse. For any protein antigen (at least 2.5 mg provided by the customer, > 80% purity in SDS-PAGE) we guarantee two positive clones or more with positive ELISA results. Our prices are on a setup fee basis. For specific pricing information, please contact us.

ServiceScreening MethodDeliverablePriceTimeline
Mouse monoclonal antibody production service ELISA 2 hybridoma clones + purified antibody (1 batch) $4,500 3-6 months
ELISA & Western Blot 2 hybridoma clones + purified antibody (1 batch) $6,500 3-6 months
Mouse monoclonal antibody production without purification ELISA 2 hybridoma clones $4,000 3-6 months
Additional hybridoma clones $200/EA 3-6 months
ELISA & Western Blot 2 hybridoma clones $6,000 3-6 months
Additional hybridoma clones $1,000/EA Add 1 week per additional clone
Protein A/G purification N/A ≥ 0.8 mg of purified antibody $250
per mouse
N/A
Ascites (not purified) N/A Ascites of one (1) mouse (2-5ml) $200 3 weeks
Ascites of five (5) mice $900 3 weeks
Ascites of ten (10) mice $1,800 3 weeks

Notes:

  1. The above prices are for the production of antibodies using antigens provided by the requestor. However, we also provide a custom protein service at an extra charge, if requested.

  2. There is a setup charge of $200 that is non-refundable when we start a custom monoclonal antibody project. The setup charge is included in the above prices.

  3. ELISA screening is also performed in (250ng/well) ELISA (general selection standard: OD value ≥ 1.0).

  4. Western Blot for screening is performed using standard cell or tissue lysates. However, if a specific cell lysate is requested, and we do not have it, the client should provide it.

  5. In special cases the timeline may be longer than indicated; you will be notified if it’s likely the case.

  6. Shipping cost is not included. (We ship the hybridoma clones with dry ice)

 

 

 

 

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