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Frequently Asked Questions

Your questions are very important to us.
If you can’t find the answer here, please don't hesitate to contact us.

 

A: It usually takes 2 to 4 days depending on where you are located.

 
 

A: You can email us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it , and you will receive a reply promptly.
Our staff will be happy to answer any questions, and all our products are guaranteed if they don't work as indicated in our datasheets.

 
 

A: You can cancel your order by logging into your account and change it in the order section, or by simply emailing us directly at This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Because all sales are final when you product is dispatched, if you haven’t received an email confirming shipment, there shouldn’t be any problems to cancel your order.

 
 

A: Please email us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it for ordering questions and This e-mail address is being protected from spambots. You need JavaScript enabled to view it for technical enquiries.

 
 

A: We do not normally offer free samples. However, if you feel you have special reasons for obtaining a sample of our antibodies or recombinant proteins, please feel free to contact us so we can find a possible solution. Buying our proteins and antibodies is risk free. We replace any of our products that don't work as indicated in our datasheets. This policy greatly reduces the need for samples.

 
 

A: We offer discount only on “bulk” purchases of five vials or more. Please contact us by email at This e-mail address is being protected from spambots. You need JavaScript enabled to view it to request a discount.

 
 

A: We cannot guarantee that an antibody will work for a species that has not been validated. However, using the sequence of the protein that was used to make the antibody you can do a sequence alignment analysis of the desired species against the antibody of interest. This will help you evaluate the probabilities of cross reaction. You can find an online sequence program called Sim at http://expasy.org/tools/sim-prot.html, and you can search protein sequences on NCBI at http://www.ncbi.nlm.nih.gov/sites/entrez?db=protein&cmd=search&term=, (or Swissprot at http://www.expasy.org/sprot/. We recommend 80% or more of the sequences to match for cross reactivity. If the antibody was generated using a peptide, an alignment should be done between the peptide sequence and its full protein sequence. We usually include the antigen sequence in our datasheet. However, if you cannot find the peptide immunogen sequence on our documentation, please email us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it .

 
 

A: Secondary antibodies should be raised against the host species of the primary antibody you are using. For example, if your primary is a mouse monoclonal, you will require an anti-mouse secondary.

 
 

A: If you require a suitable positive control, please check the datasheet since it usually includes a suggested positive control. You should use the tissue or cell line from a tested species. Not all our datasheets have a suggested suitable control, but we always try to include it.

 
 

A: Western blotting is a technique that separates proteins based on size. In general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors, so the actual band size observed may differ from that predicted. Here are some common factors:
Splice variants - splicing may create different sized proteins (mutant form) produced from the same gene
Post-translational modification - e.g. glycosylation, phosphorylation, cleavage etc, can increase or decrease the size of the protein.
Relative charge - the composition of amino acids (charged vs non-charged)
Multimers - e.g. dimers, trimers, or oligomers that have stronger affinities may have different mobility on SDS-page.

 
 

A: First we suggest testing it with our western blot protocol. Please contact us if the problem persists. We will help you find a solution and if the product was faulty we will replace it at no cost (“no cost” means just that: we pay for shipping.)
Our General Western Blot Protocol:
1. Sample: Cell lysates 30ug/well* and recombinant protein 100ng/well* loaded.
2. Sample preparation: SDS loading buffer was added to the cell lysates and recombinant protein. This solution was boiled before loading on a 15% SDS-PAGE
3. Blocking: 5% skim milk in PBS-Tween, for 1hr at room temperature, 3 times x 10 min PBS-tween washes
4. Primary antibody: Primary antibody 1:1,000*, at 4℃ overnight, 3 times x 10 min PBS-tween washes
5. Secondary antibody: Goat anti-mouse HRP conjugated 1:1,000, for 1hr at room temperature, 3 times x 10 min PBS-tween washes
6. Development: ECL
* These amounts may change according to the specifications on our datasheets. For further enquirires about a specific product you can contact us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 
 

A: The average shelf life of our proteins is about 1 month refrigerated at 4°C and 3 months at -20°C after shipment.
These periods are conservative and may be longer according to the specific protein. For a specific protein you can email us at This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Our antibodies’ shelf life is about 1 month refrigerated at 4°C and 6 months at -20°C after shipment.