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DNA Cloning Service

One mutation is defined as any combination of mutation within a 3-base frame. Our delivery package includes vector containing target gene, complete sequencing data and construct maps. Prices vary according to the length of genes. If the target gene and destination vector is not provided by the customer, there will be additional charges.

For pricing information, please contact us.

Services Deliverables Timeline
Subcloning Target gene inserted vector that the customer provides. 2 weeks
Mutagenesis Target gene inserted vector after mutagenesis. 3 weeks

 

Protein Expression & purification service (1)
Expression and Identification of target protein

ATGen has a great expertise in developing recombinant proteins using Escherchia.coli as a host. Our Protein services are charged according to the required amount of strain comparison and optimization work. The pricing is based on a setup fee for expression analysis, the number of different strains (possibly with different helper plasmid systems) and the number of different culture conditions tested. All our purified proteins have more than 95% purity on SDS-PAGE.

For pricing information, please contact us.

Services Deliverables Timeline
Expression and Identification
of target protein
SDS page image, expression levels according to strains, etc. 1 weeks
Purification 1 mg or more of purified soluble tagged protein and prices vary according to the expression patterns 1-5 weeks
Refolding 1 mg or more of refolded purified protein and prices vary according to the expression level and refolding difficulty. 2 weeks to
2 months

 

Monoclonal antibody service

ATGens monoclonal antibodies are produced in hybridoma cell lines derived from the fusion of myeloma cells and antibody-producing B-lymphocytes. We guarantee two positive clones for monoclonal antibodies developed in mouse. For any protein antigen (at least 2 mg provided by the customer, > 80% purity) we guarantee two positive clones or more with positive ELISA results. Our prices are on a setup fee basis.

For pricing and additional service, please contact contact us.

Services Deliverables Timeline
Antigen preparation 2 positive clones for monoclonal antibodies 1-5 weeks
(if not provided)
Hybridoma preparation 2 hybridoma clones 3-6 months