| Product Data Sheet | | Type | Protein | | Catalog Number | GRH3002 | | Product Name | 20kDa Growth Hormone, Human Recombinant, E.coli | | NCBI Accession No. | NP_000506 | | Description | The 20KDa variant form of human growth hormone (20KDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22K, 191 amino acid) by the deletion of amino acid residues 32-46. The physiological role of 20K hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant 20KDa human growth hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. | | Application | SDS-PAGE | | Molecular Weight | 20 kDa (177 aa), confirmed by MALDI-TOF. | | Purification | > 95% by SDS - PAGE | | Concentration | 1 mg/ml (determined by Bradford assay) | | Form | Liquid. In 88mM mannitol, 5mM phosphate, pH 7.4 | | Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. | | General Reference | Frigeri, et al (1979) Biochem Biophys Res Commun. 14; 778-82
Adelman, et al (1983) DNA. 2; 183-93
Tsushima, et al (2000) Endocr J. 47; S17-21
Horan, M.,et al(2003) Hum. Mutat. 21(4), 408-423
| | Research Category | Hormones and Cytokines,Signal Transduction,Metabolism,Differentiation and Regeneration,Aging | | PDF Brochure |  GRH3002.pdf |
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