| Product Data Sheet | | Type | Antibody | | Catalog Number | AYW0906 | | Product Name | 14-3-3 gamma antibody (clone J3H10) | | NCBI Accession No. | NP_036611 | | Description | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms,beta, gamma,epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. | | Application | ELISA, WB (Cell lysate) | | Western Blot Analysis | Cell lysates of HeLa(30ug) and mouse brain(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 gamma (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. | | Clone | Anti-human 14-3-3 gamma mAb, clone J3H10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-3 gamma protein. | | Purification | By protein-G affinity chromatography | | Concentration | 1mg/ml | | Form | Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. | | Storage | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. | | General Reference | Chen XQ, et al. (2005) Glia. 42(4):315-24 Chen XQ, Yu AC, et al. (2002) J Biochem. 23;296(3):657-63 | | Host | mouse | | Isotype | IgG2b, k | | Immunogen | Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli | | Reacts With | Human, mouse | | Usage | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~2,000. Recommended starting dilution is 1:1,000. | | Research Category | Signal Transduction | | Antigen Species | Human | | Conjugation | unconjugated | | Clonality | Monoclonal | | PDF Brochure |  AYW0906.pdf |
| |
|
