| Product Data Sheet | | Type | Protein | | Catalog Number | ATGP1576 | | Product Name | NPM1, 1-294aa, Human, His tag, E.coli | | Full Name | Nucleophosmin | | NCBI Accession No. | NP_002511 | | Description | NPM1 is a phosphoprotein which moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in NPM1 are associated with acute myeloid leukemia. Recombinant human NPM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. | | Application | SDS-PAGE | | Molecular Weight | 34.7kDa (314aa), confirmed by MALDI-TOF | | Purification | > 85% by SDS - PAGE | | Concentration | 0.5 mg/ml (determined by Bradford assay) | | Form | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.15M NaCl,1mM DTT | | Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. | | General Reference | Sagawa,F., et al. (2011) EMBO J. 30 (19), 3994-4005.
Neubauer,A., et al. (2011) Exp. Hematol. 39 (10), 1030-1042. | | Research Category | Epigenomics | | PDF Brochure |  ATGP1576.pdf |
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