| Product Data Sheet |
| Type | Antibody |
| Catalog Number | ACA0401 |
| Product Name | A crystallin A antibody (clone c9F2) |
| NCBI Accession No. | NP_000385 |
| Description | alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP). They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and the participation in the intracellular architecture. |
| Application | ELISA, WB (Cell lysate) |
| Western Blot Analysis | Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. This antibody is not shown cross-activity about Crystallin alpha B. |
| Clone | Anti-human Crystallin alpha A mAb, clone c9F2, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Crystallin alpha A. |
| Purification | By protein-G affinity chromatography |
| Concentration | 1mg/ml |
| Form | Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
| Storage | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| General Reference | Fujii N., et al., (2003) Mol. Vis. 9: 915-22 Laksanalamai P., et al., (2004) Extremophiles 8: 1-11 |
| Host | mouse |
| Isotype | IgG1, k |
| Immunogen | Recombinant human Crystallin alpha A (1-173aa) purified from E. coli |
| Reacts With | Human, Rat |
| Usage | The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500 ~ 1:2000. Recommended starting dilution : 1:1000. |
| Research Category | Heat Shock Proteins |
| Antigen Species | Human |
| Conjugation | unconjugated |
| Clonality | Monoclonal |
| PDF Brochure |  ACA0401.pdf |
